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Food particles that collide with the tentacles are trapped by mucus, and the cillia down the middle drive this mixture to the base of the tentacles.
Expression is also seen in the endoderm at base of the tentacles (Figure 5L, inset).
For dissecting scope imaging, the oral poles were cut with a scalpel near the base of the tentacles and directly observed for endogenous fluorescence.
Both anthox8a and anthox8b are expressed in the endoderm at the base of the tentacles, with anthox8a being expressed earlier, before tentacle outgrowth is initiated.
The region expressing anthox6 comes to include the endoderm at the base of the tentacles and scattered cells at the tips of the elongating tentacles (Figure 5D, inset).
In the juvenile polyp, Evx-expressing cells appear exclusively in the endoderm at the base of the tentacles (Figure 4I, inset).
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In situ hybridization analysis of adult Clytia medusae (Figure 2A) revealed that as in Xenopus [8], Poc1 mRNA is predominantly expressed in the gonads with only low expression detectable in other tissues (including at the base of the tentacle bulbs, a site of intense stem cell division [12]).
In the longitudinal view section, these two expression domains are also clearly distinct, with the first here appearing as a wide fan-shaped stained area converging to the base of the tentacle (M tcl in Figure 4A", H), in addition to a thin stripe located just above the median ridge (black arrowheads in Figure 4G-J).
The lophophore captures food particles, especially phytoplankton (tiny photosynthetic organisms), and deliver them to the mouth via the brachial grooves along the bases of the tentacles.
A brachial groove runs round the bases of the tentacles, and its own cilia pass food along the groove towards the mouth.
The cilia on the sides of the tentacles create a current that flows into the "crown" at the bases of the tentacles and exits above the center of the "crown".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com