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‡Position of the first base of the forward primer relative to the first base of the start codon.
§Position of the first base of the reverse primer relative to the first base of the start codon.
We predicted SD sequence locations within a region of 35-nt up- and downstream of the first base of the start codon for each gene following the approach of Starmer et al. (2006).
The difference is due to the fact that we are calculating the distance from the base that binds the 5'A of the 16S rRNA tail sequence 3'-CCUCCA-5' to the first base of the start codon [ 13].
A moveable aluminum barrier was used to block access to a particular T-maze arm in the Sample Phase, and was then re-positioned 25 cm from the base of the start arm to create a start area for the Test Phase.
Since the majority of the known sRNAs bind their target gene in close proximity to the RBS, we defined a subsequence of 150-nt upstream and 50-nt downstream of the first base of the start codon as the (putative) target region.
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Drip loss, cooking loss, and thawing loss were calculated as a percentage of weight loss based on the start weight of a sample.
This upper bound is based on the start of the vaccination campaign relative to the start of this study.
And based on the start of this season, Garner is not about to end his unenviable record.
You see rather than modeling his announcement based on the start of the Roman Empire, there was a much better analogy that could have been used.
Original grant agreements signed using the Euro were converted to USD based on the start date of the grant agreement [ 46].
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