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Exact(7)
One alignment must start at the first base of the read (a1 = r1).
Subsequently, each branch in the primer tree is compared with the base of the read sequence in a recursive manner.
If the length of the read is 50 bases or less, the other alignment must end on the final base of the read.
For sequence reads mapped onto the reverse (3') strand, we interpret the last base of the read as the end position of a 147 bp nucleosome.
In the next iteration, we add A (the first base of the read) and calculate and for all.
To reduce the number of possible new junction candidates generated, breseq only considers pairs of split-read alignments that meet all of the following criteria: One alignment must start at the first base of the read (a 1 = r 1).
Similar(53)
The first 14 bases of the read were trimmed and the remaining 36 bases were used for analysis.
In this evaluation, the alignment of a read is taken into account only if it has a percentage identity of ≥90% and contains ≥80% bases of the read.
Bowtie reports, for each read, one or more alignment containing no more than a few mismatches (two, by default) in the 5′-most s bases of the read.
CUSHAW2 requires the final alignment to have a percentage identity of ≥90% (default setting) and to include ≥80% (default setting) bases of the read.
The polyadenylation sites were then defined as the immediate downstream base of the reads.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com