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With pore sizes of 3 µm, the inserts allowed for the passage of stirred media through the base of the insert as seen in Fig. 3c.
We decided to use a regular pizza stone as the base of the insert; it's lighter than a base made of firebrick but will still absorb and radiate the heat necessary to set the crust.
As some endothelial cells migrate through to the base of the insert, we had to be sure that we would be able to distinguish between the endothelial and epithelial cells.
In contrast, the distinctive D653 in PfLAMMER participates in a network of hydrogen bonds involving an arginine in the β-hairpin insert; the V318 in Clk2 is replaced by T711, which itself forms hydrogen bonds with two other residues in the αE helix and at the base of the insert, rather than with D653.
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The remaining reads were combined into files based on the insert size of the library (irrespective of the prior biological treatment) and assembled using an additive multiple- k-mer (35, 39, 43, 47, 51, 55, 59, 63, and 67) approach [ 28, 29] with the Velvet/Oases assembler (Velvet version 1.1.04 and Oases version 0.1.21) [ 30].
Non-migrated cells were removed 24 h later and the base of the inserts fixed in 100% methanol and stained with 2% crystal violet solution.
a Defined as a ratio between total number of base pairs sequenced and total number of base pairs of the inserts in a given BAC clone.
EEG leads were bent into a 'u' shape and the base of the 'u' inserted into the hole so that wire contacted the dura over the parietal cortex.
The base sequence of the inserted gene in the pcDNA3.0 plasmid matched the genomic sequence of hHGF provided by GenBank.
For instance, you may want to place Easter grass or confetti at the base of the bouquet or insert additional favors into the bouquet.
Observation of the base of the leaves still inserted on the stem will show the pattern of decurrency.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com