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Using the plasmid pUC rbcLP as a template [ 21], the Chlamydomonas rbcL gene was PCR-amplified, with the first base of the coding region numbered 1, using forward (bases -169 to -144) and reverse (bases 1404 to 1438) primers.
EST assemblies were estimated to contain start codons if the length upstream of the matches (BLAST score >50) in the assemblies was greater than that between the start base of the coding sequence and the matched region of the corresponding gene.
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These changes of domains were analyzed based on the coding regions of alternatively spliced exons (See Additional file 3).
The censoring was based on the coding of these endpoints in the SEER database (alive, cancer-associated death, other death).
The calculation is based on the coding region of the element.
Our results are fairly consistent with the previous findings, mainly based on the coding regions of the genome [ 15].
The study was carried out in accordance with the standards of expected ethical behavior based on The Code of Ethics of the World Medical Association [ 26].
Includes: 1. Various texts on matters germane to ritual meat-slaughtering (including some poetry); 2. ha-Ḥibur meha-Bediḳot / Nisim ha-Kohen; 3. Responsa on the laws of sheḥiṭah, based on the Code of Maimonides.
The specific morpholino blocks protein translation of the corresponding mRNA by targeting the 5′UTR through the initial 25 bases of the coding sequence.
The sequences of the putative regulatory 5′-UTRs including 15 bases of the coding region were used for the structure predictions.
Some of the Bombyx elements possess an in-frame stop codon or a frameshift within the first 200 bases of the coding sequence, like other RTE elements.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com