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For most experiments, images were acquired by scanning in linescan mode (500 Hz) across a location at the base of the apical dendrite (Figure 2A).
We illuminated GECI-expressing pyramidal cells in hippocampal slice culture with 7 mW at the objective back aperture, and recorded the fluorescence intensity over a linescan image taken at the base of the apical dendrite (Figure 7; note that different filter sets were used for the FRET-based and the GFP-based GECIs; see Materials and Methods).
In affected neurons, there is displacement of the nucleus to the base of the apical dendrite and a markedly swollen cell body in comparison to the typical pyramidal shape of neurons in neocortical layer III (Fig. 3b).
When applicable, body length of adult Daphnia individuals was measured as the distance from the top of the head to the base of the apical spine under a dissecting microscope.
There is a significantly higher density in the tip area versus the nucleus area and in the nucleus area versus the base of the apical cells, and in the tip-oriented area versus the base of the sub-apical cells (See Additional file 2).
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Similarly, in chloronemata, the tip-oriented area contains significantly more chloroplasts than the base of the sub-apical cells (See Additional file 3).
In addition, a recent work has shown that the C. elegans CC2D2A ortholog, along with a subset of other ciliopathy proteins, localizes to the transition zone (41), a region at the base of the cilium just apical to the basal body.
This pattern corresponds to that of the basal bodies positioned at the base of the cilium at the apical pole of each ectodermal epithelial cell.
This shift in location of the symbiotic algae, from the base of the endoderm towards the apical part, was consistently seen in three independent experiments.
Internodes and leaves were counted starting at the base of the plant to the apical meristem.
These new cells migrate from the base of the crypt to the apical zone of the intestine undergoing maturation.
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