3D video-fluoroscopy is a technique for the evaluation of joint kinematics based on the alignment of 3D models of bones or prostheses and series of 2D radiographic images representing the relevant monoplanar or biplanar projections [1].
We therefore performed a site-by-site analysis of dN/dS ratios based on the alignment of the set of 136 full-length intact coding sequences used above.
First, we estimated the neutral rate of substitution based on the alignment of intronic sequences from Echinococcus vogeli and Echinococcus oligarthrus.
The nucleotide sequences of the target sites are listed in figure 2. We also determined the orthologous relationships of the target sites based on the alignment of 3′ UTRs of Drosophila Abd-A, Ubx, and Antp.
For each group of orthologs, we obtained aligned nucleotide sequences based on the alignment of the peptide sequences, which we generated with MUSCLE [76].
Initially, an alignment of nucleotide sequences of these genes were constructed based on the alignment of corresponding amino acid sequences.
A total of 34 full cDNA sequences of Actinopterygii CYP3 family genes were aligned using PAL2NAL [36] based on the alignment of protein sequences performed by ClustalX v1.83 [31].
The first step in the regular analysis protocol is based on the alignment of millions of short sequences obtained from each run.
In 2009, Wu et al. described the phylogeny of 720 bacterial taxa based on the alignment of 31 concatenated gene products.
