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Tracheas from 8-week-old BALB/c mice were diced into fragments (approximately 0.5×0.5 mm) and dispersed evenly across the base of a 25-cm culture flask.
The remaining tissue (adventitia) was then dissected into 1-mm portions, ∼25 of which were evenly distributed over the base of a 25-cm culture flask containing 2 ml of DMEM with 20% fetal calf serum (FCS), penicillin/streptomycin (400 IU/ml and 400 mg/ml), amphotericin B (5 mg/ml), and l-glutamine (27 mg/ml).
A mother dish was prepared by coating the base of a T75 cm cell culture flask with 20 ml of 0.75% (w v−1) agarose prepared in medium without serum.
Cells were removed from the base of the flasks using a cell scrapper, pooled and placed in 400 ml glass bottles and stored at −80 °C prior to the virus purification step.
The glands were cut into small pieces no larger than 1 mm3 and placed at the base of the culture flask and then incubated for 3 to 4 h, before 5 mL of keratinocyte medium (ScienceCell) was added.
A coffeepot spouts "fragrant blackness," and someone drinks deeply of a flask's "alcoholic content".
Neither does sipping Crown Royal out of a flask.
"We had a litre and a half of water and a flask of tea," said Mr Reid.
He reaches for a flask of whiskey.
Like Ken Dodd: wheeled out with a flask of tea and going for it.
During competition, when the tension got tightest, he took hits from a flask of whiskey.
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CEO of Professional Science Editing for Scientists @ prosciediting.com