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The Maximum Likelihood analysis groups the p-endosymbiont of Pedicinus badii at the base of a clade containing the p-endosymbionts Wigglesworthia and Baumannia (p-endosymbionts of tse-tse flies and leafhoppers, respectfully), some free-living bacteria, and the p-endosymbionts of distantly related sucking lice of rodents (Figure 1).
Diversification analyses found the speciation rate to be low across Anomura, and we identify 2 major changes in the tempo of diversification; the most significant at the base of a clade that includes the squat-lobster family Chirostylidae.
This models the situation in which environmental conditions shift at the base of a clade, such that the radiation of that clade takes place within a potentially novel set of environmental conditions.
We calculated the posterior probability for a WGD on a branch within the Stylommatophora at the base of a clade containing the Sigmurethra and the Orthurethra of 0.881 for Tree-10 (fig. 5).
We found that isometry was the most likely ancestral condition for the European lineage, with a single evolutionary transition to an allometric growth pattern at the base of a clade containing four of the Sardinian species.
The branch within the Hypsogastropoda at the base of a clade sister to the Strombidae and containing the Neogastropoda and several families of Littorinimorpha hereafter to be referred to as the Capulidae Neogastropoda branch had posterior probabilities of a WGD greater than 0.999 for all three trees (fig. 4).
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If the juvenile were to resolve at the base of a larger clade, thereby possible affecting several nested clades, the influence on these metrics would be magnified.
Furthermore, group C sequences did not group in the HK tree and appeared at the base of a B/D clade.
The input of a user tree, where we placed the chaetognath at the base of a protostomian clade did not result in a different topology or branch support than when a starting tree is inferred via BIONJ (for PhyML analyses).
The topology of a phylogenetic analysis of cv-lysozyme 3 cDNA (full length 663 bp, encoding an open reading frame of 187 amino acids) is also consistent with a transitional condition, as cv-lysozyme 3 falls at the base of a monophyletic clade of bivalve lysozymes identified from digestive glands.
In local tests, we compared the fit of three models for each dataset: a two-equilibrium O-U model that allows a change in environment at the base of a single clade; the single-equilibrium O-U model, in which the entire tree comprises a single environmental regime; and the Brownian motion model.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com