Sentence examples for base mutations in the from inspiring English sources

The average numbers of single base mutations in the VH genes from GC A and GC B were 7.6 and 15.8, respectively, and for clusters C and D they were 15 and 14.9, respectively.

Amplification products of five SSR markers were extracted and sequenced, corresponding repeat loci were found and locus mutations are mainly in copy number of repeats and base mutations in the flanking region.

Interestingly, we were also able to identify, that same mutation in heterozygous combination with a hemoglobin C beta globin variant having glutamic acid replaced by lysine (GAG replaced by AAG) in one compound heterozygous sickle cell patient, thereby demonstrating the ability of RNA-seq to reliably identify heterozygous single base mutations in the expressed transcripts.

A SNP results from a single base mutation in the DNA sequence and has a frequency of 1% or more in the population.

This effect was blocked by a single base mutation in the DR4 TRE; T3 did not increase luciferase activity in cells transfected the ΔDR4-tk-Luc vector.

However, transition mutations could not be denied as the most common base mutation in the EMS sphere as reported previously (Lawley and Martin 1975; Sikora et al. 2011; Shiwa et al. 2012).

We found an exceptionally high incidence of single-base mutations in the tumor suppressor genes RASSF1 and RBSP3 (CTDSPL) both located in 3p21.3 regions, LUCA and AP20 respectively.

To detect single-base mutations in the PCR products of the TCRV β gene, the single-strand conformation polymorphism (SSCP) technique was used (Orita et al, 1989).

The pGL3-Report constructs containing these 3′UTRs with 6-base mutations in the seed sequence and the constructs containing Pro-MIR199A2 Δ-2 (construct Δ-1 with 7-base mutations in the AP-1 binding site 2) were synthesized with QuikChange site-directed mutagenesis kits (Agilent Technologies, Palo Alto, CA, USA).

After quality control, sequence error rate was estimated as the percentage of single-base mutations in the rpl32- trnL region within individuals and was found to be approximately 0.13% (see Methods).

This study focused on single base mutations in BCP and preC region and the multi-mutation patterns observed in chronic HBV infection patients.