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The base medium for hFOB1.19 was a 1 1 mixture of Ham's F12 Medium Dulbecco's Modified Eagle's Medium, with 2.5 mM L-glutamine (without phenol red).
Strains were grown in a base medium composed of 100 g/L ME (Sigma-Aldrich, St . Louis MO, USA).
Strains were grown in a base medium composed of 100 g/L malt extract (ME) (Sigma-Aldrich, St . Louis MO, USA).
The strain was grown in a base medium composed of 100 g/L ME (Sigma-Aldrich, St . Louis MO, USA) supplemented with YPD.
Clostridium butyricum and Rhodopseudomonas palustris were grown on a potato starch/glucose base medium at 30 °C under continuous illumination (40 W m−2 light intensity).
During the sensor calibration, cells were incubated in the 37 °C non-CO2 incubator in 180 μl assay medium (XF Base Medium and 2 mM l-glutamine, pH 7.4).
Cooke Rose Bengal (CRB) agar base medium was used for the selective isolation of fungi.
Freshly prepared Muller Hinton Broth (MHB) medium was used as a base medium for the assay.
The other set of controls contained the fermentation base medium and the inoculum, but no glycerol.
It is cultured in the base medium: 1 1 mix of MCDB105 and 199 with the final concentration of sodium bicarbonate 2.2 g/l.
Two grams of the soil samples/two ml of water samples were enriched with 50 ml of mineral salt base medium in 250-ml conical flasks.
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