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Base line expression of immune genes in pbMEC, 24 h after changing the growth medium from SM10 to either SM Milk or SM0. Figure S4.
Base line expression of the master cytokine interleukin 6 (IL6) was found to be strongly increased by supplementing the growth medium of bone marrow cells with FCS [ 25].
The base line expression of all genes remained stable in SM10 over the entire 24 h of the experiment in SM10.
For comparison, we found that just removing the FCS from the medium (e.g. maintaining the cells for 24 h in SM0) had a weaker effect upon the base line expression of those genes, with the noteworthy exception that removal of serum reduced the base line expression of CXCL8, IL1A and IL6.
Fetal calf serum (PAN-Biotech; 3302 251110) was from a lot which we had previously validated for not enhancing the base line expression of our candidate immune genes in pbMEC.
We found that raw milk, but not pasteurized milk significantly increased the base line expression of immune genes, such as TNFα or LAP (Additional file 1: Figure S5A, B).
Similar(52)
Indeed, microarray data confirmed that amiodarone HCL-susceptible NSCs have significantly increased base-line expression of certain ion channels (Table 1, SLC2A1 and CLC1A).
One clone was selected as our reporter cell line based on low base-line expression of Venus and high expression following thapsigargin treatment (high dynamic range).
Additionally, we calculated the RFVI for each gene between the samples with the highest and lowest expression, using the base-line expression value of each gene in normal breast tissue or cell line, as described previously [ 30].
When SPA reached the values of − 45° and − 90° from base line of 0, the expression levels of vWF increased by 44 and 127% correspondingly (P < 0.0.5).
To find common expression patterns, we performed hierarchical clustering based on fold-changes in expression from a base line of 5 DAP.
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