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We observed elevated base levels of expression for all three genes already at ZT14 indicating alterations in the diurnal regulation of these genes in the absence of SHARP-1 and -2 (Figure 2C).
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Mean base levels of CYP1A expression ranged from 7.24×106 (rainbow trout) to 1.05×107 (brook trout) transcripts μg−1 total RNA.
The first is the difference between strains at control temperatures (15 °C) to establish base levels of gene expression and the second is differences between control (15 °C) and treatment (28 °C) for each of the three groups (desert, montane, and hybrids).
Three up-regulated genes (KRT34, DSC3, PSG2) and 1 down-regulated gene (RSP4Y) were chosen based on their levels of expression in the microarray study.
We grouped kiwi genes into three categories based on their levels of expression.
We also applied a filter based on detectable levels of expression in the rat brains.
Next, we grouped Asia II 3 genes into three categories based on their levels of expression.
Three clones were chosen for further study based on differential levels of expression under uninduced versus induced conditions.
RT-qPCR analysis of pre-endocrine cell populations from E15.5 to E18.5 allowed hierarchical clustering based on relative levels of expression of the genes in the Insm1/ Pdx1-HI versus Insm1/ Pdx1-LO cellsupplementaryary material Fig. S11).
We then selected 4 genes from this list based on varying levels of expression differences and location in genomic regions that either showed copy-number gain (ALDH1A3, SEMA4B) or did not (ITGBL1, NFE2L3) in PANC-1 cells.
We performed functional annotation of genes based on level of expression.
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