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bTCR rearrangements that belong to private repertoires are indicated by the base length for each patient.
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The fastq files from the MiSeq were filtered by sequence length (≥34 base and ≤39 base length for short templates, and ≥ 90 base length for long templates).
Upstream (2000 bases) and downstream (700 bases) sense-strand sequences for all genes were distributed into sequential 50 base length bins for a count of the occurrence of non-overlapping poly dA.dT tracts of N = 5, 10, 15 and 20 base length.
The Illumina machine generated 37.1 to 44.7 million pairs of 200 base length paired-end reads for each library (Table 3).
A chi-square test was performed for each base length and linkage group combination independently (results not shown).
In a compromise, partial libraries consisting of primers of 8 or 9 base length, have been designed for application in DNA sequencing [ 2- 4].
These z-scores were used to calculate dendrograms of the ten planted species, with a calculated branch length for each species based on the five traits indicating how different each species was from the others.
For example, thresholds could be chosen based on the distributions of ROH length for each species relative to the distribution of ROH length of several species together.
Values are shown for a linear polyelectrolyte, the model base-paired NA, and the actual genome length for each virus.
The number of sequences, N50 values, mean length of the sequences and total base pair length for the contigs, scaffolds and unigenes were also determined (Table 1).
Repeats with a multiple of three base lengths are candidates for genic regions due to their ability to maintain the current reading frame while potentially providing for a repetitive amino acid sequence.
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