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Marsupials also have a 9 10 base insertion within the PRE.
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The IAF-45-CD genome contains a single base insertion within this region that introduces a stop codon in a ketosynthase domain.
Twelve of these were genotyped, 2 could not be converted into TaqMan assays (deep intronic, 1 base insertions within simple repeats).
We observe significantly increased hybridization signals of single- base insertion defects in which the insertion base is placed next to a like-base.
We have found this to be most marked when the single base insertion or deletion exists within extended runs of a single nucleotide.
The average base insertion, deletion, and substitution frequencies within this flanking region were found to be 0.014%, 0.055%, and 0.080%, respectively.
At this step, we segregated any sequences containing a frame shift (single or double base gaps or insertions) within the V3 loop.
The mutations were all single base insertions or deletions within homopolymer repeats (HPR).
Interestingly, the two isolates that had base deletions within the ITS region contained a 5-bp insertion within the first intron of the MAT1-2-1 gene.
Scrib Crc mice carry a single-base insertion (3182 3183insC) within codon 947 of Scrib (Murdoch et al., 2003).
The point of insertion within the REP element is exactly between the bases occupying positions 32 and 33 of the REP sequence.
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