Exact(5)
In addition to being a robust polymerase for the amplification of large genomic regions, this polymerase also has an error rate of approximately 8.7 x 10−6 per base incorporated, and therefore introduces far fewer errors than PacBio chemistry does.
This is expressed as an increase in melting temperature (Tm), which increases by 2 8 °C per LNA base incorporated.
Tetracycline-free base incorporated into these materials was released within 10 14 days depending on polymer structure.
Bis-PNA clamps with the K base incorporated in the Hoogsteen strand showed a significant stabilization of the triplexes at pH 7 as compared to similar triplexes with PNA oligomers containing either cytosine (6.7 °C per unit) or pseudoisocytosine (1.5 °C per unit).
Upon addition of the terminating nucleotide, a fluorescent signal is emitted that is captured and denotes the type of base incorporated in the sequence.
Similar(55)
A bis-PNA with the Eag-base incorporated in the Hoogsteen strand was hybridised with a complementary DNA.
In response to the Internet fan base, New Line Cinema incorporated feedback from online users into its production, and added five days of reshooting.
Occasionally, an incorrect incoming dNTP is mispaired with the templating base and incorporated onto the 3′ end of the growing primer strand.
These criteria were set for the analysis as they were demonstrated to maximise the tradeoff between base pairs incorporated and average and maximum contig size after thorough empirical testing.
Each flow produces a light signal in each of the beads, either a very weak signal ('negative flow value', in practice being between 0 and 0.5, indicating that no base was incorporated) or a stronger signal ('positive flow value'), proportional to the length of a homopolymer run (Margulies et al., 2005).
During the PCR reaction, Adenine (A) bases are incorporated in positions complimentary to the U bases in the primer.
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