Sentence examples for base in the sequence from inspiring English sources
Next, the sequencing reads were trimmed from both sides using the modified Mott trimming algorithm to reach a Q20 score, which means that the chance that a particular base in the sequence is called incorrectly by the sequencer is 1 in 100.
In conventional Sanger sequencing, the accuracy of the call of each base in the sequence can be assessed using Phred scores, which are probability-based confidence scores (Ewing et al, 1998a; Ewing and Green, 1998b).
This method assigns a probability distribution over labels to each base in the sequence considering ChIP-chip ratio and sequence information.
Dam is a member of this family that selectively modifies the adenine base in the sequence GATC and is responsible for most of the DNA methylation in Escherichia coli [3].
The method to plot a DNA sequence is to start at the origin and take a step in the positive x-direction for a guanine base, in the negative x-direction for an adenine, positive y-direction for a cytosine and the negative y-direction for a thymine, and proceed likewise for each succeeding base in the sequence, starting each step from the end of the last one taken.
The quality of each base in the sequence was assessed with the program Phred [ 54, 55].
For every run of the HTS platform, the algorithm first generates an error model that takes into account the position of the base in the sequencing read and the identity of the two upstream bases.
The number of intervening letters, or bases, in the sequence determines the size of the segment.
More challenging scenarios arise in cases of gapped alignment of RNA-Seq reads, and with the presence of insertion or deletion of bases in the sequence of reads.
This result is also robust to the underlying sampling variance of the diversity estimates, because bootstrap resampling of the individual bases in the sequence data also demonstrates a significant correlation (median rs = −0.61; 95% CI: −0.87, −0.17).
