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The shortcoming of this method is that the residual ionic current during theDNA translocation is insufficient for direct base identification.
It enabled more accurate automated mixed base identification.
Each inner PCR reaction was performed with 32 replicates (40 μl each) using 5 μl of pooled first round template, and specific primers that incorporated a 4 base identification tag as well as a 19 base 454 adaptor sequence (Table S9).
In addition, studies involving glucose measurements usually base identification of cases on a single measurement, resulting in false-positive screens (115, 116).
This allowed us to base identification on EMG variance and thus eliminate hand- and subject-specific differences in impedance, signal amplitude, etc.
Sequences were analyzed using the Sequencing Analysis 5.2.0 software (Applied Biosystems), considering a value of 2% in the option of Mixed Base Identification.
Similar(47)
Ambiguous base identifications were sequenced again, and SNPs were identified based on the sequence reported in GenBank.
18S rRNA based identification was done using the culture identification service of Merck India Ltd.
Digest access authentication is password based identification method that allows secure user identification using passwords.
We also proposed a 3D visualization based identification method for the individual scattering object.
Relay based identification technique is adopted to estimate power system dynamics.
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