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At this step, we segregated any sequences containing a frame shift (single or double base gaps or insertions) within the V3 loop.
A 6 bp run of Ts within 50 bp of the start is expanded to include all Ts with any number of single base gaps.
Such individuals review functional and technical specifications with an eye for clinical impact, potential functionality conflicts, and knowledge base gaps.
Similar(57)
The absence of the DNA in the enzyme-DNA co-crystal structure, however, prevented us from examining the protein-DNA interactions through which DraIII recognizes the canonical 9-bp sequence with a three base gap in the middle.
A probe was retained if at least 28 of the bases matched a region in the genome with less than a two base gap within the sequence.
Blood gas analysis of the groups revealed increase in the acidosis and base gap in the S/C and S/C + EP groups versus the sham groups.
The 20 base gap was consistently found to be vulnerable, whether it was at the 3′ end or in the middle of the DNA strand.
One can see an evident similarity between the two specificities, though the alignment itself is non-trivial due to a one base gap that has to be introduced in phiEco32 consensus.
Stringency for p53 binding site probability score was set at a cutoff of 90 (out of 100) as determined by the p53MH algorithm using likelihood ratio scoring and a 14 base gap size limit between half sites.
Serum pH level was significantly decreased in the S/C group versus the sham group and acidosis occurred, base gap was developed, and a little improvement occurred in the group that received EP although being not significant.
These alignments could then be examined for mismatching bases and alignment gaps, with mismatches indicating potential miscalled bases, gaps in the ENCODE sequences indicating potential insertion errors, and gaps in the 2× assemblies indicating potential deletion errors.
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CEO of Professional Science Editing for Scientists @ prosciediting.com