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We were not able to use base frequency and codon usage based screening to identify contaminant data [ 63, 64] because contaminant sequences in our data derived from multiple genomes.
Nucleotide frequencies and codon usage were determined by MEGA (version 5.05) (Tamura et al. 2011).
Another common technique for genome classification is based on codon usage frequencies [ 43].
However, another study suggested that three-base periodicity in biological sequences had specific characteristics not reproduced in computer-generated exon-like sequences based on codon usage frequencies [ 14].
The optimization was carried out based on the codon usage frequency of the closely related strain Aspergillus niger (Abarca et al. 2004).
These frequencies reflected the strongly biased base composition and codon usage found in cytochrome b, and agreed with previous findings [ 18- 20].
Uptake sequence frequency would then be a property of acquired genes that, like base composition or codon usage, only very slowly comes to resemble that of the new host genome (Lawrence and Ochman 1997).
The relative adaptiveness of synonymous codons is deduced from the frequency of codon usage in a pre-selected set of genes relative to the expected frequency.
The frequency of codon usage in highly expressed genes defines the relative fitness values for each synonymous codon.
These charge-changing substitutions do not appear to be derived from differential neutral evolution of base composition or codon usage between Pgi-1 and Pgi-2 genes, as demonstrated by the fact that GC content and codon usage frequencies are not significantly different between Pgi-1 and Pgi-2 (GC content: P = 0.0782, Mann-Whitney U test, n = 12; rank order of codon usage: rs = 0.9509, n = 64).
This is the first study that directly compare splicing frequency with codon usage.
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