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Plants and animals showed significant differences in base frequency at each peak (Table 3).
To investigate whether this NAGNAG motif in mammalian species is also present in higher plants, we isolated 32 bp flanking the splice site for each of the delta bp values ranging from two to 10. Pictograms representing the base frequency at each position about the AA variation are displayed in Figure 6 and confirm that the most frequent sequence is a tandem repeat of CAGCAG [ 39].
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To compute the cumulative information, the base frequencies at each position in the motif-sized windows are averaged to derive a new set of base frequencies, and then the information at each position is computed using this new frequency matrix.
Therefore, for a particular codon, the method calculates the observed codon frequency and then computes its ratio to the expected codon frequency, which is derived as the product of the individual base frequencies at each codon position.
The most common way to depict a motif is by a Sequence Logo [ 25], in which the base frequencies at each position in the motif are summarized graphically by the height of each letter.
However, base frequency at SNP 4 specific for the Canina-1 and Canina-2 allele is higher (0.778) than expected (0.6; Figure 7, additional file 2).
Fig. 6 shows the observed and expected number of differences at each position of the motif between the 178 orthologous pairs between species, based on the nucleotide frequencies at each position within each one of the species.
A Perl script TILLING_parse.pl (Additional file 10) was developed to extract the called base frequency compositions at each EMS-mutable base within the target amplicon, using base qualities, by accessing pre-indexed versions of the pileup files.
Figure 2 shows that there is no significant drop in quality score values across the first ten positions of the reads, indicating good reliability of the base frequencies at these positions.
Additionally, the base frequencies at the sequence ends and the probability of tag sequences are provides to the user.
Estimates (mean and accompanying 95% credibility interval) for the sixteen sets of base frequencies at the ancestral root sequence under the context-dependent model with context-dependent model frequencies.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com