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We aim to establish the evidence base for the recognition and management of obstetric anal sphincter injury (OASI) and to compare this with current practice amongst UK obstetricians and coloproctologists.
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As the physiological significance of this behavior of AAG is elucidated, our work suggests a molecular basis for the recognition of base loops by this human DNA repair protein.
(C and D) Structural basis for the recognition of bases A and G by Ile34 and Asn34.
Open image in new window Figure 3 Structural basis for the recognition of bases adenine and guanine with new TALE codes.
(B and C) Structural basis for the recognition of bases A or G by a number of predicted or unpredicted TALE codes.
The 1.9 Å structure of dHax3 bound to the dHax3 box DNA element elucidated the molecular basis for the recognition of bases A by Ser, C by Asp, and T by Gly (Deng et al., 2012a).
The 3.0 Å structure of another TALE protein, PthXo1, in complex with DNA has further revealed the molecular basis for the recognition of bases G by Asn and A by Ile (Mak et al., 2012).
We then present fifteen crystal structures of engineered dHax3 variants in complex with target DNA molecules, which elucidate the structural basis for the recognition of bases adenine (A) and guanine (G) by reported or uncharacterized TALE codes.
The molecular bases for the recognition of biotrophs by plants outside the purview of gene-for-gene systems are still elusive.
In summary, the crystal structure of YePEPT together with structure-function studies have provided the molecular bases for the recognition, binding and specificity of charged amino acid residues at the N-terminal position of dipeptides by peptide transporters of the POT family.
This conclusion is in agreement with the crystallographic analyses of the pol κ complexes showing that this pol relies on the Watson−Crick base pairing for the recognition of the template base (99).
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