Sentence examples for base extension were performed from inspiring English sources

All reactions, including PCR amplification, shrimp alkaline phosphatase treatment, and base extension were performed in 384-well plates (ABgene) with a Puredisk pipette robot (Cybio).

The BeadChip is placed into a flow-through chamber, unhybridized and non-specifically hybridized DNA was washed away and single base extension was performed on bound primers with labeled nucleotides.

Protocol for allele-specific base extension was performed according to Agena Bioscience's recommendation.

For each SNP, both PCR-RFLP and single-base extension were performed.

However, measurements without dorsal foot extension were performed before those with dorsal foot extension.

Single-base extension was performed and the chips were scanned using the Illumina iScan.

Labeled single-base extension was performed on primers hybridized with DNA, and the hybridized DNA was removed.

The Illumina Infinium technology utilizes a bead-based approach in which 50 bp oligonucleotide probes are used to hybridize to SNP-adjacent sequences of a sample and then a single base pair extension is performed to assay the SNP base with fluorescently labeled nucleotides.

Single base primer extension was performed after deactivation of the remaining nucleotides by shrimp alkaline phosphatase.

In the first step, PCR based primer extension is performed using a phosphorylated primer, designed to extend from the boundary of the known sequence, into the flanking region.

falciparum) or 36 cycles (HBV) of single base pair extensions were performed with image capture using an Illumina (Solexa) GA2 sequencer (Illumina, Hayward, CA).