Sentence examples for base error rate of from inspiring English sources
All the four datasets have the same uniform base error rate of 2%, but have different percentages of indel errors (i.e. 20%, 40%, 60%and80%0%and80%
Sequencing was simulated with Wgsim from the Samtools package (Li et al., 2009) using the following parameters: paired-end mode with 2 × 100 bp reads, an insert size of 300 bp (std = 50) and a base error rate of 0.01.
For example, with a sequencing base error rate of 1bp per 100 bp sequenced [ 9], k erroneous k-mers will be produced, being k equal to k-mers size.
While the per base error rate of Illumina sequencing is very low (substantially less than 1%), the TCR amplicon interrogated by TCR-seq is short, such that high-copy clonotypes may show extreme coverage.
Using 29 publicly available L-SAGE libraries - that were also used in our study - and aligning the tags on the human genome sequence, the conclusion that the sequencing error rate might have been underestimated was drawn since a large number of tags did not match the genome after having taken into account the currently accepted 1% base error rate of L-SAGE tags.
Although several studies comparing 454 pyrosequencing with Sanger sequencing have shown that the per base error rates of the two technologies are similar [ 27, 30], 454 pyrosequencing has limitations.
Sogin et al. [8] showed that a number of heuristics can guarantee that per-base error rate of pyrosequencing is lower than that of Sanger sequencing while retaining more than 90% of data.
Although the per-base error rate of pyrosequencing of 16S rRNA genes is not higher than that of Sanger sequencing, the intrinsic error rate of pyrosequencing might lead to overestimation of the number of rare phylotypes.
The ability to differentiate strains by amplicon sequencing on the MinION™ was accomplished despite an observed per-base error rate of approximately 30%.
If sample s contains the precise sequence r, then the probability of a mismatched base at any position in an aligned read is equal to the per-base error rate of the sequencing technology e i, which for Illumina is taken to be 0.01, although this can vary depending on what preprocessing steps are implemented [ 31, 32].
