We found only small differences among the absorbance values for aptamers with single base differences at the 5', whereas differences in nucleotides at the stem-loop structure at the 3' end had significant effects on affinity to anti-MPT64 antibody.
We identified a single base difference at location g359 A → G on exon 1 between the reference sequence and patient's sequence.
Nuclear proteins specifically recognized one base difference at SNP-358, but not at SNP-638.
As previously shown with DNA copy number and mRNA expression data [ 28, 62], we observed location based differences at the level of methylation.
Sequenom genotyping can validate only the specific mutation called, and so lacks the ability to identify base differences at sites that were not discovered (i.e. unable to validate mutation load assays, gene burden tests), and is thus much less effective for extension.
Therefore, we use either criterion: 80% reciprocal overall or 50 bases difference at each breakpoint, to consider a prediction confirmed by assembly.
This capacity can balance our inclination to notice, and act based upon, difference at the same time that it can cultivate a sense of community.
Alteration of this nucleotide in G27 to that of 26695 restored apo-Fur dependent sodB regulation, indicating that a single base difference is at least partially responsible for the difference in sodB regulation observed among these H. pylori strains.
Probes that identify unique sequences in the oncogenic E6 region of each type were used in multiplex single base extension reactions extending at the single base difference between wild-type and competitor HPV so that each HPV type and its competitor were distinguished by mass when analyzed on the MALDI-TOF mass spectrometer.
Probes that identify unique sequences in the oncogenic E6 region of each type were used in multiplex single base extension reactions extending at the single base difference between wild-type and competitor HPV so that each HPV type and its competitor were distinguished by mass when analyzed on the MALDI-TOF mass spectrometer as described previously [ 32, 36– 36].
