Sentence examples for base coverage of the from inspiring English sources
cSNP%: the percentage of non-match base coverage of the total coverage without counting the non-crisp coverage.
Base coverage of the C. rosea genome was generated using Illumina HiSeq paired end sequencing with an insert length of 0.5 kb and read length of 100 bp using standard library preparation kits.
Figure 2 shows the base per base coverage of the reads aligned by BLAT for both the PacBio and Illumina datasets across the P. micrantha chloroplast genome, showing a more uniform coverage of genome by the PacBio RS dataset.
Based on these three counts, aligned reads' weight, and reads' base probability, three scores that reflect the likelihood of a SNP at each location in the reference are generated: cSNP%: the percentage of non-match base coverage of the total coverage without counting the non-crisp coverage.
The median per-base coverage of the input DNA sequence reads was 16-fold, and for the genomic DNA sequence reads was 8-fold, giving a genome-wide ratio of 2. We then ranked all windows from least- to most-covered by input sequence reads, normalized by the genomic read counts.
The per-base coverage of the tRNAs was computed and divided by the total number of reads and then multiplied by 100, leading to values that are comparable between samples (percentage).
The Pearson correlation between the per-base coverage of the forward and reverse strands was calculated, each time shifting the reverse strand by increments of 1 bp (beginning from a lag of 20 up to 5000 bp).
For example, the base coverage of contig NODE-882-2-0_930, one the longest of the dataset, was plotted, and the coverage per base varied from one to 22 along the 959 bp.
Using a SSAHA SNP score of 60 (reflecting a base coverage of ≥20) resulted in all of the corrections being confirmed.
The base coverage of contigs from different assemblies was generally within a range of 10 ~ 100X.
