Synthesis of RNA requires DNA as a template, thus ensuring that the base composition of the RNA faithfully reflects that of the DNA; in addition, as in DNA synthesis, all four nucleoside triphosphates must be present.
There is also a bias in base composition of the homopolymer run errors with 50% occurring in polyT runs.
This work was based on chemical information about base composition of the molecule provided by Erwin Chargaff, on data from X-ray crystallography produced by Rosalind Franklin and Maurice Wilkins, and on mechanical model building as developed by Linus Pauling.
Four base composition parameters are estimated from the average base composition of the extant sequences.
Could these correlations be spurious technical, microarray artifacts, for example by base composition of the probe?
On the contrary, we found almost no dependency between the replication times and the base composition of the segments.
To determine the length and base composition of the target sequence duplication produced by XMRV integration, ten single-clonal (isogenic) populations of XMRV-infected cells were prepared.
(GSSF) guide strand secondary structure-features, a combination of secondary structure and base composition of the guide strand proposed by Xue et al., [41].
The base composition of the second set is unskewed, representing sequences whose scores do not benefit from these base composition correction measures (μ = 0.5, σ2 = 0.03).
To take into account the base composition of the promoters a background model from a relevant set of sequences is estimated.
The HMM is trained on a subset of read-half alignments to best reflect the quality and base composition of the dataset and genome.
