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Because we mainly used the combined sequences to reconstruct the tree, we also calculated base composition for the 12 protein-coding gene set (Additional file 3).
Contigs from all three Leishmania species were aligned in ACT v4, which calculated sequence affinity with L. major and base composition for the two putative homologous sequences.
The base composition of the SSR repeat units is consistent with the base composition for the overall genome, 60 to 68% GC.
We present the transcriptome map of the mouse isochores (DNA segments with a fairly homogeneous base composition) for the three different tissues and the effects of isochores' base composition on their expression activity.
For each of the 13 protein-coding genes, we estimated base composition for the whole gene and for the third position of 4-fold degenerate codons (P4FD): alanine-GCN, glycine-GGN, leucine-CTN, proline-CCN, arginine-CGN, serine-TCN, threonine-CAN, and valine-GTN.
After sorting taxa according to base frequencies in ascending order, additional exclusion of Peripatus sp. and Sinentomon erythranum resulted in a homogeneous base composition for the 28S gene (H0: χ2 = 434.99, df = 399, P = 0.1), likewise indicating that three sub-models are suffucient to cover the taxon set.
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Analysis of gene size, exon-intron structure and base composition for these 443 predicted genes is summarized in Table 2.
Figure 3 shows a plot of the base compositions for the RdRp target region for the 3 CoV known to infect humans.
To estimate the substitution rate with respect to base composition for a region, the number of substitutions was counted and then divided by the number of appropriate sites in the ancestral sequence to obtain the substitution rate.
For the downstream region 30 to 100 nucleotides away from the poly(A) site, the base composition for plants was in the pattern U> > A> > G ≥ C but for animals was U > A> > G > C.
The position of the AT ended codons are more close to Axis1 than the GC ending codons with a concentrate distribution, indicating that the base composition for mutation bias might correlate to the codon bias.
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