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Milky Queen and was characterized by two base changes within the coding region: G to A in exon 4 and T to C in exon 5.
The locations of the base changes within the affected GLTPi sequences are shown in Additional file 1, Figure S3.
Clones containing either nonsense mutations leading to in-frame stop codons or base changes within the recombination sites which potentially impaired the subcloning efficiency, were rejected.
During our preliminary experiment, several distinctive base changes within the MAT1-2-1 sequences were found among some O. sinensis isolates.
It is perhaps because base changes within the TATA boxes of promoter elements will adversely affect transcription [ 71, 72] that only a very small number of wt MSV isolates (data not shown) have polymorphisms within the above-mentioned TATA box.
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Sequence derived from these PCR products indicated that, relative to the wild-type Myb-27 allele0 allele, the PG-1 allele contains a single base change within the myb coding region.
DNA: position of the base change within the cDNA.
The majority of splice mutations disrupt the native splice site through a base change within the invariant donor or acceptor dinucleotides (5).
No mutations were detected; however, a new polymorphism consisting of a C-to-G base change within the β-promoter was identified, with the frequency of the G allele being 0.34.
Accordingly, DNA cleavage studies using short DNA duplexes containing sequences that diverge from the consensus sequence show markedly reduced rates in the range 10 5000-fold for single-base changes within the sequence.
In contrast to the low within-species variation (0.15-0.31% nucleotide sites different) of Cochliobolus heterostrophus [ 21] and Phaeosphaeria nodorum [ 30], our comparison of 56 O. sinensis isolates detected at least 23 base changes within 877-882 nucleofides of the MAT1-2-1 sequences (2.6%), including nine nonsynonymous variations.
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