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Sequence derived from these PCR products indicated that, relative to the wild-type Myb-27 allele0 allele, the PG-1 allele contains a single base change within the myb coding region.
DNA: position of the base change within the cDNA.
The majority of splice mutations disrupt the native splice site through a base change within the invariant donor or acceptor dinucleotides (5).
No mutations were detected; however, a new polymorphism consisting of a C-to-G base change within the β-promoter was identified, with the frequency of the G allele being 0.34.
Similar(56)
Milky Queen and was characterized by two base changes within the coding region: G to A in exon 4 and T to C in exon 5.
The locations of the base changes within the affected GLTPi sequences are shown in Additional file 1, Figure S3.
During our preliminary experiment, several distinctive base changes within the MAT1-2-1 sequences were found among some O. sinensis isolates.
Clones containing either nonsense mutations leading to in-frame stop codons or base changes within the recombination sites which potentially impaired the subcloning efficiency, were rejected.
It is perhaps because base changes within the TATA boxes of promoter elements will adversely affect transcription [ 71, 72] that only a very small number of wt MSV isolates (data not shown) have polymorphisms within the above-mentioned TATA box.
The fifth gene contains a single base change within IVS2 at position 745, which results in the formation of abnormal β-globin RNA that contains an extra exon.
We have identified one limitation of MLPA, in that there can be a loss of signal in the case of a single base change within a probe-binding site.
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