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This has led to many resort-style one stop shops (particularly in the north), and more upmarket "flashpacker" hostels, such as those in the Base chain.
In step 1 of the routine, a base chain is generated containing N units of unmodified disaccharide (G0A0) (see definition of nomenclature in Materials and Methods).
Genotyping was performed using a modification of the single base chain extension assay previously described [ 15], at GlaxoSmithKline, UK.
The ASPE method was chosen over single base chain extension and oligonucleotide ligation methods for its ability to read both alleles from a given SNP in a single tube (18, 24, 25).
In space-time interval modes, this chain was separated and it was possible to identify spin domains separated from the principal base chain at the interior of the hexagonal cluster generated by fast rotation of the light particles.
The remaining polymorphisms were genotyped using Illumina (San Diego, CA, USA) GoldenGate platform (Fan et al, 2003), a single base chain extension assay modified by GlaxoSmithKline (Research Triangle Park, NC, USA) (Taylor et al, 2001), TaqMan SNP Genotyping assays (Applied Biosystems, Foster City, CA, USA; Livak et al, 1995), or sequencing.
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