Sentence examples for base calls within the from inspiring English sources
Re-examination of this region with Sanger sequencing confirmed the presence of ambiguous base calls within the expected 11-bp section.
MG-RAST's method is faster but may miss the duplicates with sequence errors (indels and wrong base calls) within the first 50 bases.
Nevertheless, there were 385,789 base differences (excluding unassigned,"N", base calls), within 43,761 unigenes, between the B. rapa-cured (or A genome-cured) and B. oleracea-cured (or C genome-cured) unigene sequences.
To validate this approach, we filtered the data to only include those repeat regions that were; a) tri-nucleotide repeats, b) were composed of high-quality reads based on the "vcf" file, and c) had no "N" base calls within 300 base pairs of the repeat region to allow for primer construction.
While Ib and Ic may be hard to distinguish in many cases, we used a workaround and determined the number of ambiguous base calls within each sequence as a rough measure for sequence quality.
All low quality base calls within contigs were masked using a PHRED base quality threshold of 16.
Libraries were 'spiked' with 20% of a balanced nucleotide library to improve base-calling within the barcode.
Libraries were "spiked" with 20% of a balanced nucleotide library to improve base-calling within the barcode region of the sequence reads.
The immediate catalyst for his downfall was Mr Hatoyama's decision last Friday to support a plan with America to relocate a United States marine base, called Futenma, within the island of Okinawa, rather than removing it elsewhere.
With the exception of wild-type calls within no-call stretches, sPROFILER does not attempt to re-examine any base calls conforming to the reference sequence because GDAS and GSEQ are conservative in making wild-type calls and thus achieve low false negative rates.
