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Image analysis, error estimation and base calling were carried out using Illumina Pipeline (version 1.4.5) to generate the sequence data.
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Base calling was carried out using the Phred program (version no. 0.020425.c).
Base calling was carried out using Phred [ 49].
Sequence data base calling was carried out using Phred [ 40].
Base calling was carried out using PHRED [ 48] and the resultant sequences were processed by removing reads of less than 80 bp in length.
For sequences where electropherograms were available, base-calling was carried out using phred [ 34].
Realignment of regions containing indels, recalibration of base qualities, and variant detection and calling were carried out using the Genome Analysis Toolkit (McKenna et al. 2010).
Genotype calling was carried out with BeadStudio software (Illumina Inc).
Copy number variation calling was carried out, as described above.
Manual calling was carried out as described in Section 3.1.
All manual calling was carried out blind to genotype calls from any of the algorithms.
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