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Image analysis and base calling were accomplished using the Illumina Pipeline (RTAVersion 1.18.54).
Data analysis and base calling were accomplished with the Illumina instrument software.
Data analysis and base calling were accomplished using the Illumina instrument software.
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Peak calling was accomplished by use of MACS [ 40] using default parameters.
The fragment size calling was accomplished with the GeneMaper software (Applied Biosystems, Foster City, California) with the help of FP Pipeliner.
Fragment-size calling was accomplished with the GeneMaper software (Applied Biosystems, Foster City, California) with the help of FPPipeliner http://www.bioinforsoft.com/.
Base calling was performed with PHRED [ 36].
Base calls were performed using RTA software.
Therefore, correct base calls were obtained.
The overall aim, namely the achievement of a secure base-calling method was accomplished by the assortment of primers that allow for full double-stranded sequence coverage of the whole mtDNA genome (in combination with previously described CR sequencing strategies).
They were accomplished.
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