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The raw cDNA sequence base calling was conducted by using Phred [12] with a cut-off score of Q20.
The generated paired-end reads (96 bp) were sorted according their indices, adapters were clipped, and base calling was conducted using freeIbis (Renaud et al. 2013).
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Sequencing was performed using a 2 × 300 paired-end configuration and image analysis and base calling were conducted with the MiSeq Control Software embedded in the MiSeq instrument.
Image analysis and base calling were conducted with the Illumina pipeline (1.8.2).
Image analysis, signal processing, and base calling were conducted using Newbler 2.3 software (454 Life Sciences Corp ,Branford, CT, USA).
The raw BES base calling were conducted by using Phred [ 48, 49] with Q20 as a cut-off.
Image analyses and base-calling were conducted using the HiSeq Control Software (HCS 1.4.5.0) and RTA component (RTA 1.12.4.0).
Following base calling, alignment and SNP calling was conducted using the ABI Bioscope vs. 1.2.1 (Applied Biosystems), with standard parameter settings for targeted resequencing.
After running a local realignment and base quality score recalibration, SNP calling was conducted using the GATK UnifiedGenotyper.
Then, variant calling was conducted using the GATK Unified Genotyper [ 11].
Roll call is conducted in an old shed.
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