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Base calling was completed using Illumina Sequence Control Software with Real Time Analysis.
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When 1500 calls had led to a complete survey being answered and all quotas met, the calling was completed.
When one telephone call is completed, a network-managing computer at the cellular base station reassigns the released frequency slot to a new caller.
When the call is completed, the music plays on.
The call is completed.
A total of 787 telephone screening calls were completed.
All phone calls were completed by a single field researcher.
Base calling was performed with PHRED [ 36].
After the whole exome sequencing was complete, image analysis, error estimation, and base calling were processed using the Illumina Pipeline to obtain primary data.
Nucleotide positions in which no base call was made or in which the forward base call was different from the reverse base call were excluded from further analysis.
PCR products were sequenced (Eurofins MWG, USA) and manual base calls and sequence trimming was completed by sequencer (5.0) BLAST [ 12] was used to identify close sequence matches (http://www.ncbi.nlm.nib.gov/BLAST/blast).nib.gov/BLAST/blast
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