Sentence examples for base call with the from inspiring English sources

The base call with the most reads is considered the primary base call.

For all variable sites, the base call with the most mapped reads was selected as the consensus using an in-house Python script.

Quality scores ≥ Q30 for 88.9% of the reads were obtained, with 91.7% of the 17.111 million clusters passing the quality filter that eliminates reads having more than one base call with chastity scores of <0.6 in the first 25 cycles.

Another useful statistic is the maximum per base-call error rate – frequency of the erroneous base-call with the highest frequency in the control plasmid -- which provided an upper bound on the combined PCR and sequencing error that occurred at a single locus.

The electrophoregrams in the ΑBI Prism 310 Genetic Analyzer were manually base-called with the Chromas Lite 2.01 software (Technelysium Pty, Tewantin, Australia) and compared with the expected sequence with the BLAST tool of PubMed.

ESTPiper incorporates the Phred program [ 7] for base calling with quality scores, which is the de facto standard program for converting DNA sequence trace files (i.e., chromatograms) generated by the Sanger method into nucleotide sequences.

First, raw sequence data were cleaned up with QCleaner software (Amelieff): low-quality reads (>20% of the base calls with a Phred score <20) were discarded; reads shorter than 32 and reads containing five or more N were also discarded; base calls at both ends with a Phred score <20 were trimmed.

Trace files were subjected to base calling with phred and data cleaning through the ESTPiper analysis tool, resulting in removal of 4,866 low-quality sequences.

Sequence reads were base called with Roche base caller version 2.5p1, and the sff files were converted to FASTQ files.

The image analysis and base calling with quality score were performed with CASAVA v1.7 (Illumina Inc. USA) according to manufacturer's instructions.

Base calling with GS-FLX System software generated 353,344 high-quality reads for the first half-run and in 785,322 reads for the second complete run.