Sentence examples for base call with an from inspiring English sources

In total, 14537 ESTs from single-pass 5' sequencing of 16149 cDNA clones (GenBank: EL680967 – EL695503) passed the quality control for high confidence base call with an average read length of approximately 600 bp.

In total, 41,248 ESTs from single-pass 5' sequencing of 45,569 cDNA clones passed the quality control for high confidence base call with an average read length of approximately 570 bp.

We also filtered out base calls with a quality value below 17, i.e. base calls with an error probability >0.02.

For variant calling using CRISP, reads with low mapping quality (<20) and base calls with a low quality score (<10) were discarded.

Sequence ends were trimmed until the first and last 10 bases contained fewer than 5 base calls with a confidence score (phred score) lower than 30.

Software Phred [ 60, 61] was used for sequence base calling, with a quality score of Q20.

First, raw sequence data were cleaned up with QCleaner software (Amelieff): low-quality reads (>20% of the base calls with a Phred score <20) were discarded; reads shorter than 32 and reads containing five or more N were also discarded; base calls at both ends with a Phred score <20 were trimmed.

Bowtie2 options are used where alignment scores are set to be equal to the number of base matches minus three times the number of base mismatches (down-weighted if they are lower quality base calls), with a gap open penalty of 2 and a gap extension penalty of 3. A staged alignment procedure is employed to accelerate read mapping and alignment.

This means that the script filtered out all variant positions that did not have at least 2 genotypes, each with 2 different base calls with a minimum phred score of 20 and within good quality context (5 bases on each side with a minimum phred score of 15).

Phred score is assigned relative to the probability of an incorrect base call, with each 10-point increase equating to a 10-fold decrease in the probability of a miscalled base.

Proprietary algorithms translate each pulse into an A, C, G or T base call with its own set of quality metrics.