Sentence examples for base call at each from inspiring English sources

As for the Illumina sequencing described earlier, combined assemblies were generated by relinearization of the reference and the consensus base call at each site was used.

Likewise, the consensus sequence is determined based on the majority of base calls at each position.

The mpileup file contains the base calls at each position, for each library.

We then extracted the base calls at each BOPA SNP location using tools from the GATK.

We found that the average error rate for each mutation category (insertion, deletion, mismatch, and ambiguous base calls) at each base position on each strand of the amplicons in the control samples was 0.22, 0.16, 0.036, and 0.014%, respectively (Fig.  1C).

We wrote a series of C programs to calculate divergence statistics (see Statistical Model of Divergence section) and to estimate LD between all SNP pairs for which there were at least 20 read pairs within a population with valid base calls at each site.

Fluctuation in homology to the parental OPV strain might be due not only to the calculation method (calculation was made on the basis of the majority-base call at each chromatogram position, and case sequences presented many mixed nucleotide positions) but also to immunotherapy.

In a worst-case scenario a base call at a given position will be unclear, requiring re-amplification and re-sequencing of the amplicon.

We also considered the ancestral state to be ambiguous if neither chimpanzee nor macaque had a base call at the site.

The properties include sample identifier, strand, read group, mapping quality, base call at a particular position, pair distance and orientation, custom tags and more.

The algorithm uses all wild-type bases within the array to determine threshold for peak to next highest intensity ratio on either strand and uses the base call at the position of interest across all arrays to determine the proportion of wild-type calls that are made on that position by GSEQ.