Sentence examples for base at a given from inspiring English sources

Depending on the identity of the base at a given SNP, a different dye (or hapten) -conjugated chain-terminating dideoxynucleotide is added to the 3'-end the oligonucleotide.

The ethical issues will be specific only for the knowledge base at a given time and for a specified production and use scenario.

Quality scores are usually assigned separately for each base at a given read position, but may be summarized as a score for the position itself (generally measuring confidence in the called base at that position).

Using a Hidden Markov Model, that study found that a useful level of complexity was four states with the probability of a base at a given position depending on the state and the previous base.

To detect significant polymorphisms, that is likely polymorphisms per se, we calculated probabilities with the same parameters, save that k = number of deviant reads supporting a particular base at a given position and P = 0.0001/3 because only one of three possible mutations was considered (fig. 1).

The deduced CDR3 nt sequences were then scanned using the Phred quality score cutoffs of 0, 10, 20 or 30 [ 24], and reads with CDR3 nt sequence containing at least one low-quality base at a given cutoff level were filtered out.

Because different bases at a given genome position may support the same polymorphism (e.g., two types of mutation are transversions), some positions contributed multiply to some vectors.

For continuity of coverage calculations, the GATK's Depth of Coverage walker was used to calculate the number of bases at a given position in the genomic alignment.

The proportions of methylated and unmethylated bases at a given position were then compared between groups of reads carrying each allele at the corresponding heterozygote site using a Fisher's exact test.

The pattern [AG]NNATGG (where N is any nucleotide, and letters in square brackets indicate alternative bases at a given position) was used, derived from published data on initiator codons from highly curated sequences [ 15].

In our view, the kpol measurements are on firm foundation as they are based on equilibrium properties, the states of ionization of acids and bases at a given pH, based on the reasonable assumption that the actual chemical transformation (bond making/breaking) is slow compared with ionization equilibration.