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Purification of [N5]-8-oxo-dGuo and DNA base analysis were performed using a Hitachi HPLC system equipped with an L-2130 pump, an L-2200 andosanpler, and an L-2450 diode array detector (systems 1 and 2).
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For quantitative estimations of catalytic activities the HPLC based analysis were performed and product formed was computed as the appearance of peaks corresponding the product in the optimized condition using modified protocol (21).
In addition, Grey relation based analysis was performed to identify the most critical parameter for optimization among three geopolymerization factors selected in this investigation, for the production of pelletized fly ash geopolymer aggregates.
Computer based analysis was performed with Image J software and calculated by the following equation: I = ∑ I/(A/N), where I is the fluorescence intensity, ∑ I is the summation of all nuclei intensity, A is the total area of the nuclei, and N is the number of nuclei used.
Since the double transfectant mutant clones Ld GLOI::HYG NEO (+/h/n) were found to contain 2.2-kb wild type GLOI allele as well as hyg and neo integrations, DNA content based analysis was performed to check the ploidy levels of the cells.
The bioinformatics based analysis was performed to obtain the structural insight of all these RuvB like proteins of R2TP complex.
The sequencing run and the base call analysis were performed according to the manufacturer's protocol with a TruSeq SBS kit v3-HS (Illumina).
All geometric morphometric methods based on Procrustes analysis were performed using MorphoJ (Klingenberg, 2011).
All the following experiments and analysis were performed based on these six selected materials.
All subsequent phylogenetic classification and analysis were performed based on the BLASTX results.
First, a component based robustness analysis is performed based on the reliability indices of the remaining elements after the removal of selected critical elements.
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