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New surface-array-based sequencing approaches that employ unitary base addition chemistries promise to make large-scale whole genome analysis a routine activity for smaller laboratories.
Dissolved phenol-C increased as base addition rate increased.
Then the synthesized cDNA was subjected to end-repair, phosphorylation, and 'A' base addition according to Illumina's library construction protocol.
Dissolved organic carbon (DOC) increased as the base addition rate increased, with the response to KOH being up to 9 times larger than to Ca(OH 2.
The approach is based on strong acid (either H2SO4 or HCl) dosage to the feed water to attain pH ∼ 4.3, followed by CO2 stripping and subsequently strong base addition to pH 9.00 9.25.
This paper reports on the development of software sensors for the on-line reconstruction of biomass and ethanol, which are based on simple feedforward neural networks making only use of conventional bioprocess instrumentation (stirrer speed, base addition for pH regulation, etc).
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Contrack International of McLean won a $63 million contract from the Army for construction services at Camp Zafar, Afghanistan, for transient and base additions.
The PacBio RS II detects single nucleotide additions in real time, directly measuring the kinetic properties of base additions during the sequencing process.
No base additions, deletions, or substitutions were unintentionally introduced into the sequence.
Nondestructive optical measurements were performed to monitor single base additions of PNA initiated from 3-aminopropyl triethoxysilanee attached to the surface of PSi films, and mass spectrometry was conducted to verify synthesis of the desired sequence.
All other reagents used for end repair, A-base addition, adapter ligation, and library enrichment PCR were purchased from KAPA Biosciences in 96-reaction kits.
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