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Increased basal tissue concentration of MELM was seen in the spinal cord of these animals [37] and this was associated with a clear reduction of release [38].
It is possible that the EV values simply reflect basal tissue specific expression variations of genes and not the variability of their expression under different cellular conditions.
The results showed that the 37 °C treatment induced 2-16 fold increases in miR397a, miR477b, novel262, novel177, novel482, and novel188 expression levels in shoot basal tissue.
Such a pattern was observed in the plots of Principal Component 4 (and probably also Principal Component 2, if the strong influence of the most basal tissue location is temporarily disregarded).
The human colorectal carcinoma cell line Caco-2 was obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in basal tissue culture medium (DMEM) supplemented with 8% foetal calf serum, 1% penicillin, and 1% streptomycin (all Gibco BRL, Paisley, UK) in a humidified 5% CO2, 95% ambient air atmosphere at 37°C.
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Activities of invertases and sucrose synthase were stimulated by HS treatment in apical tissues and inhibited when HS were applied to basal tissues.
They then chose the 200 most highly expressed genes (100 in apical and 100 in basal tissues) between the developmental stages studied.
Biopsy specimens from the distal duodenum were obtained during routine endoscopy and were immediately frozen (basal tissues) or incubated as described later and were subsequently stored in liquid nitrogen until DNA or RNA was extracted.
To investigate the effect of high temperature on viral titer with respect to miRNA pathways in different tissues, the expression levels of miR397a, miR477b, miR3627-5p, miR5519, novel262, novel177, novel345, novel482, novel566, novel188, novel197, and novel241 were also quantified in the shoot basal tissues treated at 24 °C and 37 °C using qRT-PCR.
However, these studies were performed on transformed cell lines or LPS-stimulated hepatocytes and it remains to be seen whether the modulation of mTOR under basal conditions in primary tissues causes significant changes in cellular redox-homeostasis.
To test for possible compartmentalization of the symbiont infections within host individuals (i.e. physical separation of multiple infections), we dissected basal trunk tissue, anterior ovisac, posterior ovisac/root, outer ovisac sheath, and inner ovisac tissues from 21 of the larger specimens (7 worms from dive T1119, and 14 worms from dive DR12).
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