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This hypothesis is supported by previous findings on Merlia normani, (Poecilosclerida: Merliidae), a demosponge species known to possesses forms with and without a calcified basal skeleton [ 43].
These spherulites increase in size and are moved proximally to the hyper-calcified "basal skeleton" where they fuse together and further increase in size by epitaxial growth along the c-axis of the aragonite crystal.
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No pair of these intra- and extracellular scl-CAs are closely related to CAs involved in formation of the carbonate basal skeletons of the demosponge Astrosclera [ 3], supporting the view that CA-mediated carbonate formation evolved independently in calcite-spicule forming Calcarea and demosponges with a basal carbonate skeleton.
Jackson and co-workers [ 3] reported the involvement of CA in the formation of the basal carbonate skeleton of the demosponge Astrosclera willeyana.
Neither scl-CA1s nor scl-CA2s have phylogenetic affinities with the Astrosclera CAs, which have been shown to be involved in the formation of the basal carbonate skeleton of this demosponge species [ 3].
While several other sponges can form such basal carbonate skeletons in addition to or in place of their siliceous spicules, only sponges from one of the four currently recognized sponge classes, the calcareous sponges (Class Calcarea), are capable of producing calcite spicules, which is a synapomorphy of this class [ 17].
These at the point of conjuncture form the glenoid fossa, which articulates with the basal component of the skeleton of the limb.
Silica skeletons of basal stem showed epidermal-like cells, 30-40 μm wide and 100-300 μm long, with heavily silicified cell walls and approximately equidistant punctate deposits of silica within the walls which were suggestive of the expected locations of plasmodesmata.
In other silica skeletons of basal stem the sections were characterised by many small punctate deposits of silica, <1 μm across, while the stomata, ca 40-50 μm in diameter, were more numerous, only lightly silicified and many appeared to be linked in pairs.
Fig. 11 Skeleton of a basal ornithopod from Wucaiwan, similar to G. wucaiwanensis.
a The skull of the basal tyrannosaur Guanlong wucaii; b skeleton of Guanlong wucaii (the crest on the skull of this specimens is broken off); c skull of the basal ceratopsian Yinlong downsi, from above (left) and the left side (right).
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