Once mitotic spindle and centrosomes are properly positioned in an unequal manner, a set of adaptor proteins, such as Miranda and Pon, are localized to the basal side of cells (Knoblich 2010; Bonifacino 2014).
Exocytosis-vesicles at the basal side of cells as well as their abundant mitochondria, free ribosomes and rough endoplasmic reticulum reflect a high metabolic activity and suggest an active role in nutrient processing before their release into the blastocoel.
Moreover, the blebs disappeared faster at the apical side of cells compared to the basal side of cells.
Engagement of the ECM in all three dimensions, compared to only at the basal side of cells as in a conventional culture, significantly influences cell behavior.
However, in the cells that were fixed after 60 min, some blebs were observed that contain P-FAK at the basal side of cells (Fig. 3b ABC).
Although HCs in the mature retina are always found located at the apical side of the INL with tangential processes extending from the apical side of the cell, immature HCs sometimes migrate into the photoreceptor layer and have tangential processes extending from the basal side of the cell (5/22 cells; Fig. 3A, 25.0 h, 30.0 h).
Cno apically localizes with the Bazooka/Par-3 in neuroblasts, and was also found to be essential for proper distribution of cell-fate determinants on the basal side of the cell; importantly, failure of the determinants to basally distribute resulted in misorientation of the mitotic spindle.
The rate of neurite elongation was dependent on the ECM species, that were pre-coated beneath the cell soma, suggesting the ECM stimulated the basal side of the cell soma and affected the outgrowth process of the neurite.
The objective focus was aimed near the basal side of the cell.
Only a single Z section was bleached and imaged, typically the basal side of the cell contacting the coverslip.
