Salivary samples were obtained in basal and pentagastrin-stimulated conditions (6 mg/kg s.c).
The tissue samples were obtained from basal root region of eight 70-day-old plantlets.
Once a suspected diagnosis of DKA was made, the initial laboratory blood samples were obtained for basal metabolic parameters and complete blood count with differential; urinalysis was performed for blood and urine ketones by both the nitroprusside and enzymatic methods.
Mean pulmonary artery pressure (PAPm), central venous pressure (CVP) and mean systemic arterial blood pressure (SAPm), cardiac output, arterial and mixed venous blood samples were obtained in basal conditions, hypoxic period previous embolism, after started NO inhalation and 5, 15, 30 and 45 min after embolism induction.
Placental tissue samples were obtained and syncytiotrophoblast basal plasma membranes were prepared as described previously [26] for analysis of the GLUT1 glucose transporter, the only functional glucose transporter at the maternal-fetal interface.
Following an overnight fast, blood samples were obtained to establish the basal levels of glucose and insulin, and a frequently sampled intravenous glucose tolerance test (FSIGTT) was performed as described elsewhere [81] [83].
The following samples were obtained from each group: basal sample (day 0), after 2 or 7 days of fermented milk feeding, and 12, 18, 22 or 28 days after tumour cell inoculation.
These samples were obtained from various cortical regions, hippocampus, basal ganglia, and spinal cord in four neurodegenerative diseases (AD, PD, HD, and ALS).
In some experiments, urine samples were obtained from the same mice under basal conditions (with unrestricted access to food and water) and after 18 h of food and water deprivation.
The samples were obtained at three different time points: before (basal), at the end of the race (race), and 24 hours after the race (24 h).
