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The basal sample was used as calibrator.
For the basal sample, and 12 days after tumour cells injection, mammary glands were removed.
This pair had a genetic distance of 0.60, and consisted of a cambium sample and a basal sample.
They were weighed daily and three mice per group were sacrificed by cervical dislocation at 0 (basal sample) and 5 days after re-nutrition.
The electrolytes concentration (sodium, potassium, and chloride) measured postrace was similar to the basal sample, despite the event being performed in high condition of humidity and temperature.
A basal sample of blood was taken at the randomisation visit, and protein was measured in a 24 hour urine sample before participants started the consumption of bars.
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TNBC (Basal) samples were classified by the PAM50 molecular subtyper as previously published55, 56.
Following a 120-minute basal sampling period, sham and infected animals received either intraportal saline or N[omega ]-nitro-L-arginine (L-NNA; 37 [mu ]g [middot] kg[minus ]1 [middot] min[minus ]1) infusion for 180 minutes.
Basal samples (200 µl) were drawn at 0 and 120 minutes after mounting and replaced with clean Ringer's solution, and tracer concentration was determined using fluorescent intensity of basal samples.
For the Wang dataset [14] we used the relative transcript levels of ER (probe set ID 205225_at), PGR (208305_at), ERBB2 (216836_s_and and KRT5 (201820_ato to identify basal samples.
A stable baseline level of dopamine (±10%) was reached at 4 hr after insertion of the probe [49], [71], followed by the collection of 3 basal samples.
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