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After a basal record of fluorescence in Cl−-containing HEPES buffer, cells were perfused with a chloride-free buffer to induce a chloride efflux from the cytoplasm and subsequently exposed to HEPES buffer to allow the chloride readmission.
Figure 1 shows an original basal record of LV pressure and +dP/dt max and -dP/dt max of LV pressure in a control rat and a diabetic rat.
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When applied, the electrical stimulation started immediately after the basal recording (T0) and lasted always for ten minutes.
After 5 min of basal recording of arterial and left ventricular pressure, 1 and 15 μg/kg of dobutamine were administered intravenously in a random sequence.
Following 1 h of basal recording in which the animal breathed air, a transparent respiratory hood was placed over the newborn's head and loosely tied (18, 19).
After LV pressure basal records, the LV function was recorded during a volume overload protocol performed by an injection of saline (0.27 mL/min/kg) during 3 minutes adapted from Souza's study [ 13].
Afterwards, a basal recording of both integrated C-reflex responses and wind-up activity prior to the intrathecal administration of recombinant IL-1β (2 ng/10 μl, equivalent to 11.4 nM) or saline (10 μl).
Test pulses were given at 0.1 Hz and neurons were clamped at −65 mV during basal level recording and LTP recording.
Thus, the tetrahedral sheet accounts for larger basal spacing recorded for cinnamate intercalation in ZLH host.
The electrode in the apical chamber was set at zero while the electrode in the basal chamber recorded the resistance/voltage.
However, the mesothelial AI in pretreated samples was still significantly greater than the basal levels recorded in pure mesothelial cultures.
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