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Basal media were prepared as described previously [5].
The control groups were injected with basal media, without cells.
Lipase activity was determined for submerged fermentations using (a) basal media (diamond); (b) basal media with acid pre-treatment coconut dregs (circle); (c) basal media with alkali pre-treatment coconut dregs (square); (d) basal media with acid pre-treatment coconut dregs and coconut oil (triangle); and (e) basal media with un-treated coconut dregs carbon.
In an initial trial, five different basal media (salts and vitamins) viz.
Neonatal chondrocytes were the most proliferative and adult chondrocytes had the lowest ALPSA in basal media.
Vegetative inoculum (Ve-I) was prepared by inoculating 5 mL basal media with 1 × 107 spores under same aerated culture conditions for 10 h which was used for inoculating 95 mL basal media.
A time course of the PYT production was studied using the basal media (pH 5.5) in triplicates.
A variety of complex culture media and techniques developed from different basal media have been reported with alternate success.
Biofilm structure developed with mineral basal medium appeared as a monolayer, but in complex basal media cell aggregates were appreciated.
Basal media consists of 0.01% MgSO4·7H2O, 0.1% KH2PO4 and 0.5% peptone was supplied with 1.5% of acid, alkali or untreated coconut dregs as a sole carbon source.
The lipase activity was further reduced to only 26 U/mL when B. stratosphericus was grown in the basal media without coconut dregs.
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