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Basal levels of cell death were not observed to differ significantly between WT and PAR1−/− mice.
Preliminary results indicate that the basal levels of cell envelope stress responses are also lower following overexpression of yhbW, the other multicopy suppressor that we isolated.
Basal levels of cell death with and without light exposure are listed in the figure legends.
All experiments compared the cell death induced with the basal levels of cell death in the cultures.
As shown previously in Figures 3A, B and 6A, B, C2-ceramide and a prolactin blocking antibody each alone increased basal levels of cell death.
In Figures 5A C, prolactin alone (100 ng ml−1) had no effect on basal levels of cell death in either the MCF-7, T47D or Hs578T cells.
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Fluorescent images of PC12 cells not exposed to magnetic nanospheres had a very low basal level of cell death (Fig. 5a).
This can be regarded as basal level of cell death.
Hormonal adjuvant therapy of breast cancer is overall tumoristatic with cell death balancing a basal level of cell proliferation [ 24].
On the other hand, overexpression of RARα1 had no impact on the cell cycle distribution, indicating that the endogenous level of RARα1 is optimal for supporting the basal level of cell cycling.
When equal numbers of live bax +/+ and bax -/- cells were seeded and mock-treated, bax -/- cells produced more colonies, consistent with the much lower basal level of cell death in these cultures (see Figure 4).
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